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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2023: 95.56
Index Copernicus ICI Journals Master List 2023 - IJCMAS--ICV 2023: 95.56
For more details click here
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Original Research Articles Volume : 10, Issue : 3, March, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
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Int.J.Curr.Microbiol.App.Sci.2021.10(3) : 2195-2200
DOI : https://doi.org/10.20546/ijcmas.2021.1003.276


Amplification of Housekeeping Genes in Magnaporthe grisea causing Blast Disease in Foxtail Millet

M. Dhivya1*, S. Thiruvudainambi1, G. Senthilraja2, N. Senthil3 and C. Vanniarajan4
1Department of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625 104, TN, India 2Regional Research station, Tamil Nadu Agricultural University, Vriddhachalam 606 001, Tamil Nadu, India 3Department of Plant Molecular Biology and Bioinformatics, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India 4Department of Genetics and Plant Breeding, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai 625 104, Tamil Nadu, India
*Corresponding author
Abstract:

The genus Pyricularia (anamorph)/Magnaporthe (teleomorph) causes blast disease on a broad range of plants including rice, foxtail millet and other species of the family Poaceae. The infected leaves of foxtail millet with typical spindle shaped lesions of light tan in colour, dusty gray appearance with necrotic borders were collected from various regions of Tamil Nadu during 2017 and 2018 and the pathogen was isolated from the samples. The collected isolates were named as TNFxM1 to TNFxM32. All the 32 blast isolates were characterized by their colony morphology and spore morphology. Amplification of housekeeping genes viz., Actin, ß- tubulin, and Calmodulin were done using the specific primers at 336 bp, 536 bp and 512 bp respectively.


Keywords: Magnaporthe- housekeeping genes- PCR

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How to cite this article:

Dhivya, M., S. Thiruvudainambi, G. Senthilraja, N. Senthil and Vanniarajan, C. 2021. Amplification of Housekeeping Genes in Magnaporthe grisea causing Blast Disease in Foxtail Millet. Int.J.Curr.Microbiol.App.Sci. 10(3): 2195-2200 doi: https://doi.org/10.20546/ijcmas.2021.1003.276
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license

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