Pleurotus cystidiosus (Oyster mushroom) was grown using various agro-wastes substrate as: sugarcane (ScB), wheat straw (WS) and rice straw (RS), The ScB, RS and WS agro-wastes substrates were selected as the most favorable lignocellulosic sources. P. cystidiosus can efficiently degrade the agro-wastes and grow at wide range of temperatures. The effects of growing the type of Oyster mushroom on agro-wastes as substrates were chosen through evaluating the parameters in the growing mycelium including carbohydrates, protein, fiber, moisture, fat, ash, and minerals. The WS showed the highest yield (97% Bio efficiency), maximum crude protein (23%), and phosphorus content of (890 mg/ 100 g of dry mushroom). The ScB recorded minimum moisture (88%) and crude fiber content (9%). However, the maximum moisture (91%) and highest fruiting bodies size (10 cm) are recorded on RS cultivation. The combination of substrates formula ScB+ WS+ RS expressed the highest carbohydrate content (58 %), ash (7.1%), phosphorus (585 mg/ 100g of dry mushroom), and calcium (320 mg/ 100g of dry mushroom) content. Finally, we concluded that the P. cystidiosus of Upper Egypt can be cultivated under temperature variation and substrates mainly on WS, then RS, and ScB. The aim of the current research was studying the effect of the agro-wastes substrates and some nutritional conditions on the mycelial growth of P. cystidiosus, during winter season in the Upper Egypt.

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