International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:9, September, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 1324-1333

Genotypic Diversity among Indian Isolates of Sclerotium rolfsii Sacc. [Teleomorph Athelia rolfsii (Curzi) Tu & Kimbrough] Based on ITS Region of Ribosomal DNA
R.K. Sharma1*, I.U. Dhruj2, T. Radhakrishnan3 and S. Desai4
1Agricultural Research Station, S. D. Agricultural University, Ladol – 384540, India
2Office of the Directorate of Research, Junagadh Agricultural University, Junagadh – 362001, India
3Directorate of Groundnut Research (ICAR), Junagadh – 362001, India
4Central Research Institute for Dry land Agriculture, Hyderabad – 500059, Telangana, India
*Corresponding author

Twenty isolates of Sclerotium rolfsii Sacc. collected from different hosts and locations of India was studied in relation to genomic DNA amplification through internal transcribed spacer (ITS-PCR) analysis. These isolates of S. rolfsii showed variation at rDNA level which was revealed through ITS1-5.8s-ITS 4 primer series. The consensus primers (ITS 1 and ITS 4) amplified a region of the rRNA gene repeat unit, which includes two non-coding regions designated as ITS 1 and ITS 2 and the 5.8s rRNA gene. It was carried out with ITS-PCR analysis based on their molecular size and a genetic distance was created using Rf value. Out of 20 isolates, six reproducible polymorphic bands were obtained using the above ITS1-5.8s-ITS 4 primer series. The ITS amplified region of 5.8s rRNA gene yielded an ITS fragment of 490−699 bp length in all the 20 isolates of S. rolfsii. Among 20 isolates, six isolates showed amplification of a double band whereas the remaining isolates showed amplification of a single band. These six isolates also showed a length variation in this region. Isolates of groundnut were same almost in their size. The results showed that the ‘ITS types’ within isolates were almost always phylogenetically distinct. There was no clear correlation between ITS-based phylogeny and isolate origin.

Keywords: Sclerotium rolfsii, Ribosomal DNA Internal Transcribed Spacer (ITS) region, Polymerase Chain Reaction (PCR), Molecular characterization, Isolates

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How to cite this article:

Sharma, R.K., I.U. Dhruj, T. Radhakrishnan and Desai, S. 2018. Genotypic Diversity among Indian Isolates of Sclerotium rolfsii Sacc. [Teleomorph Athelia rolfsii (Curzi) Tu & Kimbrough] Based on ITS Region of Ribosomal DNA.Int.J.Curr.Microbiol.App.Sci. 7(9): 1324-1333. doi:
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