National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Twenty isolates of Sclerotium rolfsii Sacc. collected from different hosts and locations of India was studied in relation to genomic DNA amplification through internal transcribed spacer (ITS-PCR) analysis. These isolates of S. rolfsii showed variation at rDNA level which was revealed through ITS1-5.8s-ITS 4 primer series. The consensus primers (ITS 1 and ITS 4) amplified a region of the rRNA gene repeat unit, which includes two non-coding regions designated as ITS 1 and ITS 2 and the 5.8s rRNA gene. It was carried out with ITS-PCR analysis based on their molecular size and a genetic distance was created using Rf value. Out of 20 isolates, six reproducible polymorphic bands were obtained using the above ITS1-5.8s-ITS 4 primer series. The ITS amplified region of 5.8s rRNA gene yielded an ITS fragment of 490−699 bp length in all the 20 isolates of S. rolfsii. Among 20 isolates, six isolates showed amplification of a double band whereas the remaining isolates showed amplification of a single band. These six isolates also showed a length variation in this region. Isolates of groundnut were same almost in their size. The results showed that the ‘ITS types’ within isolates were almost always phylogenetically distinct. There was no clear correlation between ITS-based phylogeny and isolate origin.
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