International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:9, September, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(9): 430-443

Isolation and characterisation of Fusarium Resistance Gene Candidates in Zingiber spp. of North East India
N.C. Lenbi1, 2, Huidrom Sunitibala Devi1* and Pratap Jyoti Handique2
1Institute of Bioresources and Sustainable Development (IBSD), Imphal, Manipur, India
2Department of Biotechnology, Gauhati University, Assam, India
*Corresponding author

Rhizome rot and yellows caused by Fusarium oxysporum f. sp. Zingiberi, is a serious a serious soil-borne disease of ginger and found to be prevalent in the north-east region of India. Most of the plant disease resistance (R) genes encode a highly conserved nucleotide binding site and leucine-rich repeat structure (NBS-LRR). These structure can be used to isolate candidate genes for Fusarium resistance in Zingiber spp. Degenerate oligonucleotides which have been designed to recognize conserved coding regions of known resistance (R) genes from different species of plant in literature were obtained to target PCR to amplify resistance gene analogs (RGAs) from Zingiber spp. of north east India. PCR amplification from genomic DNA yielded a group of fragments of approximately 350bp and 600bp DNA sequences. From the results we can conclude that Zingiber RGCs belong to the CC-NBS-LRR class of proteins and no TIR-RGCs were found in the study. Although RGCs were detected with degenerate RGC primers and the RGC-specific primers we designed. The expression of the disease resistance seems to be very low or absent in the Zingiber species found in the region.

Keywords: R-genes, Resistance gene candidates (RGC), CC-NBS-LRR class, Fusarium oxysporum f. sp. Zingiberi

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How to cite this article:

Lenbi, N.C., Huidrom Sunitibala Devi and Pratap Jyoti Handique. 2018. Isolation and characterisation of Fusarium Resistance Gene Candidates in Zingiber spp. of North East India.Int.J.Curr.Microbiol.App.Sci. 7(9): 430-443. doi:
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