National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Rhizome rot and yellows caused by Fusarium oxysporum f. sp. Zingiberi, is a serious a serious soil-borne disease of ginger and found to be prevalent in the north-east region of India. Most of the plant disease resistance (R) genes encode a highly conserved nucleotide binding site and leucine-rich repeat structure (NBS-LRR). These structure can be used to isolate candidate genes for Fusarium resistance in Zingiber spp. Degenerate oligonucleotides which have been designed to recognize conserved coding regions of known resistance (R) genes from different species of plant in literature were obtained to target PCR to amplify resistance gene analogs (RGAs) from Zingiber spp. of north east India. PCR ampliï¬Âcation from genomic DNA yielded a group of fragments of approximately 350bp and 600bp DNA sequences. From the results we can conclude that Zingiber RGCs belong to the CC-NBS-LRR class of proteins and no TIR-RGCs were found in the study. Although RGCs were detected with degenerate RGC primers and the RGC-specific primers we designed. The expression of the disease resistance seems to be very low or absent in the Zingiber species found in the region.
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