National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In an endeavour of exploring alternative energy sources to petroleum based fuels, bio-ethanol (ethanol derived from biomass) is considered as the most promising renewable fuel because of its potential to cut greenhouse gas emissions by 86% and higher octane (ability to resist compression) rating than gasoline. Present investigation was aimed at bio-conversion of paddy straw to ethanol using partially purified fungal cellulases. A variety of soil samples were tested for the presence of cellulolytic fungal strains using enrichment culture technique. Fungal strains were selected based on the diameter of clearance zone on carboxymethylcellulose (CMC) agar plates. Selected strains were tested for the cellulase viz., exoglucanase and endoglucanase activities before and after partial purification. Out of the 10 selected cellulolytic fungal isolates, F-1 isolate has the highest 0.42 IU/ml exoglucanase and 1.66 IU/ml endoglucanase activities. Enzyme production was maximum in Mandels and Sternberg medium containing delignified paddy straw as carbon source at 30°C after 7 days’ incubation with0.66 and 2.52 IU/ml exoglucanase and endogluanase activities, respectively. Ammonium sulfate saturation at 50-55% followed by dialysis resulted in the partial purification of crude cellulase enzyme with2.8 and 2.1 folds’ increase in exoglucanase and endogluanase activities, respectively. Hydrolysis of delignified paddy straw using partially purified enzyme obtained from F-1 isolate resulted in 63.7% solubilization of polysaccharide fraction at 500C after 4 h reaction time.
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