|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
230 soil actinomycetes were isolated from the coffee plantation of Western Ghats, Karnataka, India along the altitudinal gradients and depths. 24 morphologically distinct species were obtained based on the aerial spore chains and by the sequencing of the 16S rRNA gene. The strains were assigned to the order Micrococcales, and novel orders Pseudonocardiales ord. nov., Streptomycetales ord. nov., and Streptosporangiales ord. nov. The frequently isolated genus was Streptomyces, along with rare actinomycetes Actinomadura, Spirillospora, Actinocorallia, Arthrobacter, Saccharopolyspora and Nonomuraea. This study is the first report on Nonomuraea antimicrobica as a soil actinomycete. Diversity studies on the distribution of soil actinomycetes indicated significant differences (P< 0.05) among Shannon diversity indices of sample group depths along the slope. An attempt was made to correlate the total actinomycete count with soil parameters, by PCA based multiple linear regression (MLR) which significantly correlated (P<0.0001) with pH, moisture, available nitrogen and phosphorous. About 91.6% of the isolates screened were found to be potentials for enzymatic activity. The most active enzyme producer Streptomyces sp. MH470335 produced 18.51, 1.53, 6.92, 5.62 and 5.15 U/ml for cellulase, pectinase, xylanase, amylase and protease respectively. Plantation soil actinomycetes showing enzymatic activities in vitro may indicate the potential for their use as stabilized biocatalysts.