Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Login as a Reviewer


See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 5, Issue:9, September, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 530-539
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.059


Investigation of the Action of the Anolyte after Different Storage Times on the Gram-negative Bacteria
Teodora P. Popova1*, Toshka Petrova1 and Stoil Karadzhov2
1University of Forestry, Faculty of Veterinary Medicine, 10 Kl, Ohridski Blvd., Sofia 1756, Bulgaria
2Bulgarian Association of Activated Water, Kutuzov blvd, 39, 1619 Sofia, Bulgaria  
*Corresponding author
Abstract:

Studies were carried out to determine the sensitivity of pathogenic Gram-negative bacteria (Salmonella enterica ATCC and Pseudomonas aeruginosa strain â„–450) to electrochemically activated 3% aqueous sodium chloride solution (anolyte) in concentrations of 100%, 50%, 25% and 12,5% immediately after preparation (fresh) and after storage at room temperature for 7 days, 21 days and 28 days. As a control was used the disinfectant Virkon S in concentrations of 1%, 0,5%, 0,25% and 0,125%. It was found that the suspension of P. aeruginosa with density of 106 cells/ml was killed after 2 minutes under the influence of all tested concentrations of the fresh anolyte. The same strain in concentration of 108 cells/ml was killed within 2 minutes by 50% and 100% fresh anolyte, but the lower concentrations had such effect after 5 - 10 minutes. The anolyte storaged 7 days had similar action. After 21 and 28 days the anolyte in all tested concentrations had fast bactericidal activity on P. aeruginosa in suspension of 106 cells/ml (in 2 minutes), but in such of 108 cells/ml single cells remained viable even after 10 minutes. S. enterica in both tested suspensions - of 106 and 108 cells/ml was inactivated within 2 minutes by all examined concentrations not only of the fresh anolyte, but of these stored for 7, 21 and 28 days. Virkon S in all tested concentrations killed within two minutes P. aeruginosa and S. enterica in both examined densities.


Keywords: Electrochemically, sodium chloride, anolyte, Pseudomonas aeruginosa, Salmonella enterica, antibacterial activity.

Download this article as Download

How to cite this article:

Teodora P. Popova, Toshka Petrova and Stoil Karadzhov. 2016. Investigation of the Action of the Anolyte after Different Storage Times on the Gram-negative Bacteria.Int.J.Curr.Microbiol.App.Sci. 5(9): 530-539. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.059
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

Citations