Enterococci are normal inhabitants of human colon and biliary tracts. In recent years, emerged as much important opportunistic pathogen of human diseases, causing life threatening hospital acquired infections. They have gained great significance globally, because of their increased resistance to the widely used antimicrobials and Vancomycin.  The objective was to analyze antibiotic resistance pattern of enterococci from clinical samples. The current study was carried out in the department of Microbiology, Chengalpattu medical college from January 2015to December 2015. Enterococci isolated from the clinical samples like urine, blood pus and samples were confirmed and antibiotic susceptibility pattern were observed. Enterococcus spp. isolated in the present study from various clinical specimens was 120.  Enterococcus faecalis (93.3%) and  Enterococcus  faecium (6.7%) were exclusively isolated species. Antibiotic susceptibility pattern revealed maximum resistant to Penicillin (91%), followed by Ciprofloxacin(81%), Ampicillin (69%), Doxycycline (62%)  and high level Gentamycin (48%).  Among the isolates 3% were resistant to Vancomycin. The rational and appropriate  usage of antimicrobials in the community and health care centers will minimize the emerging  multidrug and Vancomycin resistant strains of enterococci. Any therapeutic management should be based on the antibiotic policy of the concerned center and susceptibility pattern of the isolates from the clinical specimens.

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