International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:9, September, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 400-404

Extended Spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolated from Burn Wound Infections
Khulood Abdulkareen Hussein*, Zainab Alag Hasan, Utoor Talib Jassim,  and Abdulameer Abdullah Al-Mussawi
College of Nursing, University of Basra, Basra, Iraq  
*Corresponding author

To detect the extended spectrum beta lactamase (ESBL) producing Klebsiella pneumoniae isolated from burn infections using phenotypic methods, and then testing their resistance against antibiotic. Forty two bacterial isolates were collected from infected burn wounds at burn center in Al-Fayhaa General Hospital in Basra city/Iraq, then cultured on MacConkey agar to detect Klebsiella, and then cultured on CHROM agar orientation to identify K. pneumoniae. Ninteen  of K. Pneumonia isolates were screened for antibiotic resistance, including:  Amoxicillin (AX), Doxycycline (DO), Gentamicin (CN), Amikacin (AK),and screening it for ESBL producing. 19 (45.23%) isolates identified as K. pneumoniae, 15 (78.9%) of them were ESBL producer and 4 (21%) were ESBL nonproducer. All the nineteen isolates were resistant to Amoxicillin, 11 isolates to Doxycycline, 16 isolates to Gentamicin, and 18 isolates to Amikacin, while 15 isolates were ESBL resistant. Increasing prevalence of ESBL producing K. pneumoniae in nosocomial infections, especially in infected burn wondand this adds difficulty in curing post burn infections and may increase the rate of post burn mortality and morbidity. High number of isolates were antibiotics resistant, and this reflect the importance of implementation of susceptibility tests for successful treatment.  Using CHROMagar media gives better results and faster in identifying ESBL.

Keywords: Extended-spectrum β-lactamases (ESBL),Klebsiella pneumoniae, β-lactamases,burn infections, CHROMagar.

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How to cite this article:

Khulood Abdulkareen Hussein, Zainab Alag Hasan, Utoor Talib Jassim and Abdulameer Abdullah Al-Mussawi. 2016. Extended Spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae isolated from Burn Wound Infections.Int.J.Curr.Microbiol.App.Sci. 5(9): 400-404. doi:
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