International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:9, September, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 311-323

In Vitro Studies on the Influence of Different Concentrations of Growth Regulators on Economically Important Orchid, Vanilla planifolia
Vijaykumar Biradar1*, Ashashree Inamdar2, Aswini Shamse and M.S. Patil3
1PG Studies and Research Centre in Biotechnology, Karnatak College,Bidar-585 401, Karnataka, India
2Department of Biotechnology, Karnatak College, Bidar-585 401, Karnataka, India
3Department of Botany, Karnatak College, Bidar-585 401, Karnataka, India  
*Corresponding author

Vanilla is propagated by stem-cuttings. Collection of stem-cuttings from the mother plants to arrest of growth, development and yield. In order to meet the demand for propagates, the rapid regeneration method of this species is essential. Micropropogation of Vanilla planifolia has been reported through callus culture, protocorn, root tips and axillary bud explants. Even though few reports are available on in vitro propagation, the protocols are complicated, hence in this report a simple, economical, rapidly multiplying and highly reproducible protocol for large scale micropropogation is made.  In this work, the protocol developed for shoot tip culture of Vanilla Planifolia on Murashige and Skoog’s medium supplemented with different growth regulators. In this experiment the shoot induction is studied on MS medium supplemented with different concentration of BAP, in which it is showing average leaf production on BA 0.5 mg/liter. we have also studied effect of different concentration of BAP & coconut water (CW) on  average leaf formation in which BAP 1.0 mg/liter with 1.5%  coconut water showing average leaf formation and also one of the MS basal medium which is supplemented with different growth regulators that is IAA, Kinetin, BAP, D-Biotin, Ca-Pentatrenate, is also showing the maximum leaf formation.

Keywords: Vanilla planifolia, Micropropagation, Growth regulators, BAP, Coconut water, Callus,MS medium.

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How to cite this article:

Vijaykumar Biradar, Ashashree Inamdar, Aswini Shamse & M.S. Patil. 2016.  In Vitro Studies on the Influence of Different Concentrations of Growth Regulators on Economically Important Orchid, Vanilla planifolia.Int.J.Curr.Microbiol.App.Sci. 5(9): 311-323. doi:
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