National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present investigation was carried out at Centre for Plant Molecular Biology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India. The main objective of this research is to retain the recipient parent genome outside the target gene with help of SSR markers. For this purpose,background screening was done in the selected four introgressed lines (BC3F3) viz.,UMI 79/936-C1-7-7-7-46-2 ,UMI 79/936-C1-7-7-7-80-17 ,UMI 79/936-C1-7-7-7-92-1 and UMI 79/936-C1-7-7-7-92-7 with major QTLs for resistance to sorghum downy mildew in order to estimate the recovery of the genome of the recurrent parent. A total of 51 SSR markers, which showed distinct alleles between the parents, were selected for the genome wide background analysis.The graphical genotyping of the BC3F3SDMresistant introgressedlines revealed that the complete recovery of UMI 79genome was present in chromosome 3 in UMI 79/936-C1-7-7-7-46-2 and UMI 79/936-C1-7-7-7-80-17.The complete recovery of UMI 79genome was present in chromosome 7 in UMI 79/936-C1-7-7-7-92-1and UMI 79/936-C1-7-7-7-92-7.The genotype UMI 79/936-C1-7-7-7-46-2 has revealed that the 92.45 per cent (maximum recovery) of the genome were derived from the recurrent parent UMI 79,genotype UMI 79/936-C1-7-7-7-80-17 andUMI 79/936-C1-7-7-7-92-7 showed that 89.68 per cent and 80.6 per cent ofrecovery respectively.
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