National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present Study Analysis of genetic diversity of commercial tomato varieties using molecular marker viz. RAPD was carried out at carried out at Department of Plant Biotechnology SDMVM’s College of Agricultural Biotechnology, Georai Tanda, Paithan Road, Aurangabad (M.S.), 431001 with an objectives 1.Extraction of DNA from commercial tomato varieties. 2.To analyzed genetic diversity of commercial tomato variety using molecular marker viz. RAPD. The information about the genetic diversity will be very useful for proper identification and selection of appropriate parents for breeding programmed including gene mapping, and ultimately for emphasizing the importance of marker assisted selection (MAS) in tomato improvement worldwide. PCR based molecular marker RAPD allows the rapid detection of DNA polymorphisms from many individuals samples in order to avoid erratic amplifications, good quality of genomic DNA free from contaminations and standardization of PCR reaction concentration is a perquisite for developing strategies for crop improvement programs in future. Out of those two primers, both of shows polymorphisms. In the result the OPA-11 primer shows 25% polymorphisms and the OPG-19 primer shows 66.66% polymorphisms.
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