With emerging immunocompromised status, fungal infections are taking the leading role as the causative agents in most of the dreadful infections. Candidemia is becoming a leading cause of healthcare associated infections nowadays among pediatric population. A Retrospective observational study was done at a tertiary care hospital, New Delhi. 60 candida species from blood culture were analysed for speciation and prevalence in the hospital. Blood culture was performed by Bact/Alert 3D system [Biomerieux,France]. Chromogenic agar and Vitek 2 YST identification card (bioMerieux, France) was used for speciation. Blood culture results showed that Non albicans candida (73.3%) species were the majority compared to candida albicans (26.6%). Candida tropicalis (33.3%) was the most common species isolated followed by C.glabrata (21.6%). Majority of the candida species were isolated from pediatric ICU (53.3%) compared to pediatric ward (46.6%). This study shows the clinical significance and mycological shift of candida species in blood culture of pediatric population with predominance of non albicans candida.

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