The plants have investigated in the search for new substances against microorganisms resistant to current pesticides and antibiotics. The various organic extracts of A.spinosus were evaluated for invitro antifungal  activity against some yeasts including two yeasts viz. Candida albicans SRTCC (3971) and Saccharomyces cerevisia SRTCC (1781) and three fungi viz. Aspergillus niger SRTCC (1073), Fusarium oxysporium SRTCC (611) and Aspergillus flavus SRTCC (1072). The in vitro antifungal activity was evaluated at different concentrations (1000 μg/ml, 500 μg/ml, 250 μg/ml, 100 μg/ ml) by agar disc diffusion method. A.niger were the most susceptible fungal strain while F.oxysporium was the most resistant one. The results were compared with the standard antifungal . Through this research was showed the presence of antifungal constituents in extracts of Amaranthus spinosus L., revealing its potential against these pathogenic strains tested.

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