The present study was aimed to know the efficacy of probiotics in bacteria biochemical characterization (Nitrosomonas and Nitrobacters pecies) and pathogenic bacteria (Pseudomonas species) were analysed water quality in fish treated with pond probiotics. Two types of commercial probiotics were used i.e., Aqua gut (fish) as feed probiotic-treatment-2 and Nitro-PS+ Micro-Pro (fish) as soil and water probiotics treatment-3 (manufactured by Asian Bio Tech, Hyderabad, Andhra Pradesh, India).The fishponds stocked with catla (Catla catla), rohu (Labeo rohita) and grass carp (Ctenopharyngodon idella) were selected and are designated as control pond (T1), Treatment-2 and Treatment-3.  Treatment 2 and 3 were treated with probiotics control pond without probiotic.  The present study revealed that the use of probiotics in experimental pond the beneficial bacteria load ((Nitrosomonas and Nitrobacter species) is drastically increased but decreased the level of pathogenic Pseudomonas species. The isolated probiotic bacteria was identified by morphology and biochemical characterization.

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