National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Pseudomonas fluorescens isolates NLR-B1, NLR-B2, NLR-B3 were tested in dual culture against R. solani among which NLR-B3 isolate was found to be more antagonistic in vitro against Rhizoctonia solani than other two isolates. Sclerotial germination was tested after incubating in NLR-B3 bacterial suspension in the concentrations from 10-4 to 10-8 CFU/ml, there was no inhibition in sclerotial germination at 10 min incubation at all concentrations of bacterial suspension, whereas at 20 min incubation, inhibition of sclerotial germination was 73.33 per cent at 10-4 concentration, 43.33 per cent at 10-5 concentration, 30 per cent at 10-6 concentration and there was no inhibition of sclerotial germination at 10-7 and 10-8 concentrations. At the incubation period of 30 min. inhibition in sclerotial germination was 53.33 per cent at 10-4 concentration, 36.67 percent at 10-5 concentration, 30 per cent at 10-6 concentration. Similar inhibition of 13.33 percent was observed both at 10-7 and at 10-8 concentration. Per cent inhibition increased with increase in concentration of bacterial suspension at 20 and 30 min incubation periods.The bacterial suspensions at the earlier concentrations were added to the soil and incubated for 10 days after which the sclerotia were retrieved and tested for their viability, the inhibition of sclerotial germination was 56.67 per cent at 10-4 concentration, 36.66 per cent at 10-5 concentration and 16.66 per cent at 10-6 concentration. However at lower concentration of 10-7 and 10-8 there was no inhibition of sclerotial germination. There was a significant increase in per cent inhibition of sclerotial germination with increase in concentration of the bacterial suspension.
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