National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Unexpanded leaf rolls were used as explants for callus induction with different concentrations of mutagenic chemicals (sodium nitrite and ethylmethane sulfonate). The effect of these mutagenic chemicals on callusing, shooting and rooting was studied. Callus induction was observed in different concentrations of sodium nitrite (3 mg l-1, 5 mg l-1 and 7 mg l-1) and EMS (0.6 µM, 0.8 µM, 1.0 µM) along with controls. Among all the treatments 3 mg l-1 (T4) SN and 0.6 µM EMS (T10) in 2009T5 were found to be the best for callus induction traits. The treatments responded with minimum number of days for callus initiation, maximum number of explants induced callus with high callus induction frequency and high callus size.
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