Anti-Vibrio Activity of Parkia javanica: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation on Four Vibrio cholarae Strains

Susmita Saha; Jhinuk Basu Mullick; Parichita Ray Choudhury; Partha Saha; Debaprasad Chakraborty; Samir Kumar Sil

doi:http://dx.doi.org/10.20546/ijcmas.2016.508.058

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Original Research Articles Volume : 5, Issue:8, August, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Int.J.Curr.Microbiol.App.Sci.2016.5(8): 538-544

DOI: http://dx.doi.org/10.20546/ijcmas.2016.508.058

Anti-Vibrio Activity of Parkia javanica: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation on Four Vibrio cholarae Strains

Susmita Saha¹, Jhinuk Basu Mullick¹, Parichita Ray Choudhury¹, Partha Saha¹, Debaprasad Chakraborty² and Samir Kumar Sil¹*

¹Molecular Genetics and Cell Physiology Lab., Department of Human Physiology, Tripura University, Suryamaninagar, West Tripura-799022, India
²Department of Pathology, Tripura Medical College, Hapania, West Tripura-799014, India

^*Corresponding author

Abstract:

Parkia javanica, a medicinal plant, of North-East India, was screened for anti cholera activity against 4 Vibrio cholarae strains. Fresh stem barks of Parkia javanica was collected and crude methanol extract of Parkia javanica (MEPJ) was prepared by Hahnshin rotary evaporator. The minimum inhibitory concentration of MEPJ was evaluated on Vibrio cholarae strains by serial dilution technique. 10mg/ml of MEPJ is the IC₁₀₀ dose for Vibrio cholarae strains. MEPJ also showed bactericidal activity against all four Vibrio cholarae at the same concentration. The lag phase become extended after treatment with IC₅₀dose of MEPJ. Intracellular reactive oxygen species (ROS) was increased (40% - 60%) in presence of MEPJ indicating ROS mediated mechanism of growth inhibition.

Keywords: Parkia javanica, Vibrio cholerae, growth curve, ROS.

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How to cite this article:

Susmita Saha, Jhinuk Basu Mullick, Parichita Ray Choudhury, Partha Saha, Debaprasad Chakraborty and Samir Kumar Sil. 2016. Anti-Vibrio Activity of Parkia javanica: Studies on MIC, MBC, Growth Curve Analysis and ROS Generation on Four Vibrio cholarae Strains.Int.J.Curr.Microbiol.App.Sci. 5(8): 538-544. doi: http://dx.doi.org/10.20546/ijcmas.2016.508.058

Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Original Research Articles Volume : 5, Issue:8, August, 2016

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