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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An attempt was made to find out natural and cheaper methods for the control of vegetable pest. Locally available plant C. procera was used and a good methodology was developed to extract the secondary metabolic compounds from the latex. Secondary metabolic compounds extracted in form of different polarity fractions and in purity of >90% as single pure compound in each fraction, showed tremendous activity in vitro test against secondary stage juveniles of Meloidogyne javanica. The extracted fractions were found toxic to juveniles by different capabilities between concentrations ranged from 50-300 ïg ml-1. The activity of compounds was found dependent on concentration, time of exposure and the polarity of the extracted fractions. Two highly lipophilic nature of fractions having clear solubility in solvents such as hexane, dichloromethane were found extremely active by giving larval mortality of test nematode by more than 90% at their 250 ïg ml-1 concentrations, after an exposure period of 48hr. whereas, quick and absolute mortality in these fractions was observed at 300 ïg ml-1 concentrations. Ethyl acetate soluble fraction also gave more than 80% mortality of test nematodes at 250 and 300 ïg ml-1 concentrations, after an exposure period of 48hr. A high concentration viz., 1000 ïg ml-1 of the fraction especially derived from polar part of latex and found soluble in methanol was required to bring about 80% juvenile mortality of the test nematode that to after an exposure period of 72hrs. The EC formulations developed from non-polar fractions during their bio-efficacy test in pots also revealed a good control of nematode gall formation on roots of brinjal and chilli which on turn found to enhance yield of both of the crops to a great extent. As compare to control, the three formulations especially developed from hexane, dichloromethane and ethyl acetate soluble fractions were found to control nematode galls on root nearly to 50% on brinjal and 40% on chilli at their concentrations of 1.25g/pot. The formulations at this concentration also gave a yield enhancement of 70-100% and 50-60% respectively in case of brinjal and chilli as compare to control.
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