National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bambara groundnut (Vigna subterrenea Verdc. L) is an African pulse crop, cultivated mainly as protein source. The crop is most suitable for arid and semi-arid tropics and tolerant to saline soils. Low Productivity of pulses in the country is attributed to many biotic stresses which are ranging from plant ideotype to biotic and abiotic stresses. Among the various diseases, yellow mosaic virus (YMV) disease in pulses and also causes yield losses in Bambara groundnut. Hence the present study was conducted to screen 55 stabilized mutant lines of M6 generation irradiated with gamma irradiation. Five SSR primers were designed using the pick primer tool from the NCBI website for the RGA primer RGA 1F-CG/RGA 1R sequence reported in Vigna mungo due to its sequence similarity found in many of the Vigna species. Hence the same sequence was used to design primers. These heterologous primers were used to screen the isolated and stabilized mutants of Bambara groundnut. However all the designed primers were unable to amplify the mutant lines which infers that either all the lines lack resistance or these markers are not sufficient to identify the linked marker DNA to yellow mosaic virus disease resistance gene in Bambara groundnut. Hence an array of heterologus probes with large number of mutants needs to be screen.
Citations |
 |
 |
 |
 |
