International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:4, April, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 2609-2615

Efficiency of Crude Extract of Ocimum sanctum, Ocimum gratissimum and Ocimum basilicum Leaves against Bacterial Pathogens
Veena Krishnamurthy, S.C. Chandrashekar* and M. Sudeep Kumar
Department of microbiology, Sri Siddhartha Medical College, Tumkur-572107, India
*Corresponding author

Antibiotic resistance and the clinical efficacy of existing antibiotics have become a major cause of concern. The indiscriminate use of commercial antimicrobial drugs to treat infectious diseases has also sometimes been associated with negative effects on the host such as hypersensitivity, immune response and allergic reactions. Hence there is need to develop alternative antimicrobial drugs to treat infectious disease from various sources including plants. Ocimum have shown strong antioxidant and antimicrobial activity. Ocimum plant is an annual and perennial herbal plant. The aim of the present study was to investigate the antimicrobial activity of leaf extract of different species of Ocimum to different bacterial agents by disc diffusion methods. Strains of Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Shigella dysentriae and Salmonella typhimurium were selected to study the antimicrobial activity of leaf extract of Ocimum species. The antimicrobial activity was test by Kriby Bauer disc diffusion method against three different concentrations viz., 250µg, 500µg and 1000µg. The antimicrobial activity was maximum at the disc concentration of 1000µg/ml, followed by 500 µg/ml and 250µg/ml. the zone of inhibition was the highest 1.5cm for Escherichia coli, 1.4cm for Shigella dysentriae and 1.3 cm for Salmonella typhimurium, and 1.2cm for Bacillus subtilis and Staphylococcus aureus. The antimicrobial activity of plant extract in our study was better for Escherichia coli than other bacteria. The results of the present study support the medicinal use of plant for treatment of various ailments and determination of appropriate concentration for therapy can be useful.

Keywords: Antimicrobial, Efficacy, Disc-diffusion, Ocimum

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How to cite this article:

Veena Krishnamurthy, S.C. Chandrashekar and Sudeep Kumar, M. 2018. Efficiency of Crude Extract of Ocimum sanctum, Ocimum gratissimum and Ocimum basilicum Leaves against Bacterial Pathogens.Int.J.Curr.Microbiol.App.Sci. 7(4): 2609-2615. doi:
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