National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An effective protocol for in vitro proliferation and multiplication of Gerbera cv. Partrizia was established during 2015-17. Among the two stages initially used for culture initiation, capitulum explants cultured at immature stage responded better than the mature stage. Best establishment of immature capitulum explants and more number of quality shoots was obtained on modified MS medium supplemented with 10 mg/l BAP and 1 mg/l IAA. Half strength MS medium containing NAA (0.5 mg/l) + IBA (0.5 mg/l) was best for root induction. The regenerated plantlets were efficiently hardened in glass jars filled with vermiculite + agropeat (1:2) moistened with half-strength MS medium salts and covered with polypropylene lids, thereafter plants were successfully transferred to the greenhouse with good survival. This protocol can produce 32,768 to 1,65,880 vigorous, healthy and true to the type plants/year from a single capitulum.
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