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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:3, March, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(3): 2118-2130
DOI: https://doi.org/10.20546/ijcmas.2018.703.249


An Exploration of Rhizobium from Green Gram Root Nodules in the three Agroclimatic Zones of Karnataka, India
Gurubasayya Kallimath1* and C.R. Patil2
1Department of Agricultural Microbiology, College of Agriculture, Dharwad, Karnataka, India
2Department of Agriculture Microbiology, AC, Dharwad, UAS, Dharwad, Karnataka, India
*Corresponding author
Abstract:

An investigation was carried out to isolate plant growth promoting rhizobacteria from (PGPR) the rhizosphere, endorhizosphere and root nodules of green gram soil samples collected from three different agro climatic zones of Karnataka. A total of 29 rhizobial isolates from nodules isolated in that based on morphology and Gram reaction these strains were tentatively grouped as Rhizobium (29). All isolates were evaluated for eight plant growth promotional traits under in vitro. In particularly isolates 2DWRR and 9DWRR fixed respectively 5.07 and 4.46 mg N2 g-1 of carbon utilized respectively. Isolate 12UKR produced 13.61 µg ml-1 IAA. Isolate 10DWRR produced 7.72 µg GA per 25 ml respectively. Under in vitro studies some isolates inhibited plant pathogens tested. Isolate 2UKR recorded the maximum zinc solubilization (7.00 mm) followed by 2DWRR. Two isolates of Rhizobium namely; 2DWRR and 9DWRR were efficient in traits like nitrogen fixation and nodule formation on green gram. The study helped to identify isolate 2DWRR, 9DWRR as potential PGPR strains for green gram.


Keywords: Rhizobium, Nitrogen fixation, Antagonistic activity, HCN and siderophore

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How to cite this article:

Gurubasayya Kallimath and Patil, C.R. 2018. An Exploration of Rhizobium from Green Gram Root Nodules in the Three Agroclimatic Zones of Karnatak.Int.J.Curr.Microbiol.App.Sci. 7(3): 2118-2130. doi: https://doi.org/10.20546/ijcmas.2018.703.249
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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