National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Marker assisted indirect selection of resistant genotypes using linked markers has been reported as an effective breeding approach for developing yellow mosaic disease resistant cultivars. Seven genotypes of greengram with different genetic makeup showing resistance reaction to yellow mosaic virus (YMV) were selected along with a susceptible one for molecular confirmation with respect to YMV reactions. These were screened with PCR-based technique employing primer pairs to validate efficiency and reliability of identified markers loci (CYR1 and YMV1). Both CYR1 and YMV1 marker showed consistent polymorphism with respect to disease reaction in seven resistant genotypes. CYR1 was produced an allele size of approximately 90 bp. which concluded that seven genotypes (OBGG-2013-8, OBGG-2013-12, OBGG-2013-11, OBGG-2013-16, OBGG-2013-20, OBGG-2013-21 and OBGG-2013-39) have yellow mosaic virus resistance gene and both the markers are efficient and ubiquitous for genotyping of YMV reaction. OBGG 2013-20 was an YMV resistance and high yielding line which can be used as YMV donor or can be released as a variety after further multi-location evaluations.
Citations |
 |
 |
 |
 |
