National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Functional associations between phytobacteria and cultured plants have often been successful; some authors suggest that co-cultivation of bacterial strains with in vitro cells or tissues, seems to be one of the appropriate approaches for mass propagation of plants. This study analyzed the effect on growth and development of a desert plant Fouquieria splendens callus, co-cultivated with an endophyte bacterium. From all the obtained results, F. splendens callus evaluated at 19 and 45 days showed no visible damage, discoloration and diminish of growth comparing between co-cultivated and no co-cultivated conditions. Particularly the plant regulators added to the medium and the presence of the endophyte bacteria, favored the increase in callus biomass of this plant species. Scanning electron microscopy analysis of the selected callus showed morphological changes induced in co-cultivated conditions with the development of an extracellular matrix, giving an early shoots development, as a differentiation event. In this work, the establishment of co-cultives between F. splendens callus and the streaked endophyte on culture medium showed that it could be a complex network of responses between the plant growth regulators present in culture medium and the release of phytohormones produced by the phytobacteria.
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