National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
We succeeded in large volume artificial cultivation of Cordyceps militaris, using silkworm live pupa obtained by aseptic breeding. The appearance (shape, color, and size) of fruiting bodies of cultured bacteria was almost the same as that of wild one. We investigated the antiproliferative inhibitory effect on eleven human cancer cells in vitro using the water extracted fraction obtained from this fruiting body homogenate. As a result, this soluble fraction effectively inhibited the growth of some cancer cells in a concentration-dependent manner. In particular, the effect on leukemia U937 cells was remarkable, but there was almost no effect on bile duct adenocarcinoma TYBDC-1 cells or pancreatic KP-3L cancer cells. In addition, almost no effect was exerted on three normal cells (human hepatocyte (NHH), human mammary epithelial cells (NHME), human epidermal melanocytes (NHEM)) at the same concentration. Therefore, it was revealed that the human cell proliferation inhibitory effect of the C. militaris water extract fraction greatly differs in susceptibility depending on the type of cancer cell under the concentration range not affecting normal cells. Furthermore, the anti-cancer effect of C. militaris was associated with an induction of cell cycle arrest and caspase-mediated apoptosis. This fact suggests that some compounds contained in metabolic fluids of these mushrooms can be lead compounds leading to the development of anticancer drugs with very few side effects.
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