National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Fish is a relatively inexpensive source of protein and an important source of foreign exchange in many regions of world and water is the physical support in which they carry out their life functions. Genetic study of bacterial isolates found in the guts, gills and skin samples of catfish obtained from EbuteIbooro landing site of Yewa River was carried out using Random Amplified Polymorphic DNA – Polymerase Chain Reaction Techniques. Morphometrics, weight, length of the fishes were recorded and water quality parameters of the water body were also recorded. Bacterial isolates were obtained from samples grown in culture medium using Nutrient agar. Subculturing was done in order to obtain the pure isolates. Five RAPD primers were used, a total of number of 62 markers were generated with 54 being polymorphic and 8 being monomorphic. Two main groups were observed from the dendrogram derived from the RAPD results. Group 1 consists of 1 organism while group 2 consists of 19 organisms. Group 2 were further divided into sub groups with each sub group having its own constituent of organisms. Haemolysis test carried out on this bacteria isolates to determine their pathogenicity showed that 12.5% of the organisms indicated complete haemolysis, 37.5% indicated incomplete haemolysis while the remaining 50% indicated no haemolysis. The results show that RAPD fingerprinting is one of the best molecular tools for identification.
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