National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Pseudomonas aeruginosa MCCB 123 is a potent producer of enzymes such as β-1,3 glucanase, LasA and LasB proteases, which have been identified to have greater significance in the biotechnology sector. However, pathogenicity assessment of bacteria is essential for its industrial survival. Pathogenicity was assessed by a panel of virulence assays such as the presence of type III secretion toxin genes, motility assays, biofilm formation, adhesion and invasion assays on Hep-2 and HeLa monolayers and antibiogram profiling. Analysis of the major cytotoxic exoU gene revealed the absence of the gene, thus confirms the non-cytotoxic phenotype of the bacterium. P. aeruginosa exhibited three types of motilities viz., swimming, swarming and twitching motilities and proved to be a moderate biofilm producer with a 3.10±0.52-fold increase in the optical density at Abs570 when compared to control. Antibiogram suggested the possibility of antibiotic treatment as an effective method for eradication of its biofilm. The non-pathogenic nature of the bacterium suggests industrially viability of this organism for the production of biotechnological relevant enzymes.
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