Today livelihood exploration of endophytic microorganisms as a new source of antibiotics is very intensive, especially from medicinal plants. Piper betle, a green betel plant, is a medicinal plant. So that bacterial isolates from Piper betle are likely to produce antibacterial compounds. This study aims to screen the endophytic bacteria of green betel plants that live wild in HPPB forest, Andalas University. The leaf sampling was done by purposive random sampling, bacterial isolation was done by spread plate method on tryptic soy agar (TSA). Screening of antibiotic-producing endophytic bacteria was done by paper dish method. The results showed that isolation of endophytic bacteria using TSA medium obtained 86 isolates. Screening of antibiotic-producing bacteria was obtained by eleven isolates with six isolates having antibiotic activity against E. coli and S. aureus test bacteria; one isolate was only active against E. coli alone and four isolates were only active against S. aureus. Endophytic bacterial, isolates BES-2 has the largest inhibitory zone diameter (18.58 mm) and includes strong category antibiotics.

Download this article as