National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An attempt was made to isolate 62 strains of fluorescent pseudomonads from rhizosphere of soybean collected from two soybean growing areas viz., Belagavi and Dharwad districts of Karnataka. These isolates were subjected for morphological, biochemical and functional characterization. All the isolates were gram negative rods. Colour of the colony varied from light green, green, dark green to light orange. The isolates showed fluorescens under UV light. Out of 62 isolates 36, 24, 52, 36, 39 isolates were positive for starch hydrolysis, arginine hydrolysis, oxidase test, denitrification and gelatin liquification respectively. These isolates exhibited wide variations for their ability to utilize different carbon sources. With respect to MPS, 52 isolates showed MPS activity. Forty seven isolates were positive for HCN production, in which 10 of the isolates were strong HCN producers. All the isolates were positive for siderophore production, which ranged between 6.00 to 36.37mm. Whereas, IAA and GA production ranged from 39 to 28.03 and 0.5 to 18.52 mg /25 ml of broth respectively. All the 62 isolates were assayed for their antagonistic activity against Sclerotium rolfsii and Colletotricum truncatum using dual plate technique. Out of 62 isolates, 51 and 38 were inhibitory to S. rolfsii and C. truncatum respectively.
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