International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:12, December, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(12): 3808-3816

Exploring Microbes for their Cellulolytic and Lignolytic Enzyme Activity for Manure Preparation
Neethu T. Madhavan1, K.G. Patel1, Trupti K. Vyas1* and Sree Ganesh2
1Food Quality Testing Laboratory, Navsari Agricultural University,Navsari – 396450, Gujarat, India
2Department of Genetics and Plant breeding, Navsari Agricultural University, Navsari- 396 450
*Corresponding author

In recent years, production of voluminous agricultural waste creates a major problem and is of environmental concern. Hence, there is great urge to adopt a reuse of agricultural waste. The objective of this study was to isolate cellulose and lignin degrading bacteria which are able to degrade agrowaste and convert agrowaste into green manure. Total of 103 bacteria were isolated. All these isolates were screened for their cellulase production by CMC agar plate assay. Out of which 51 bacterial isolates showed zone of clearance on CMC agar plates. 14 bacterial isolates which showed higher clearance zone on CMC agar plate were further selected for quantitative screening. Isolate 53 produced higher cellulase enzyme 0.636 U/ml. For screening of lignin degradation, ABTS plate assay was used for laccase enzyme. Out of all the 103 isolates, 10 showed positive result on ABTS agar plate. Among these 10 isolates, 4 isolate selected for further quantitative assay for Lignin Modifying Enzymes (LME) i.e., laccase, LiP and MnP. Isolate 72 showed higher laccase (453.6), LiP (95.0) and MnP (526.35) enzyme. Growth was also monitored upto 96 hrs of incubation during enzymatic studies. Two potential cellulolytic (isolate 53) and lignolytic (isolate 72) isolate were identified by 16 rDNA as Bacillus licheniformis C1 and Bacillus sp. L2, respectively.

Keywords: Biodegradation, Bacillus, Cellulase, Laccase, LiP.

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How to cite this article:

Neethu T. Madhavan, K.G. Patel, Trupti K. Vyas and Sree Ganesh. 2017. Exploring Microbes for their Cellulolytic and Lignolytic Enzyme Activity for Manure Preparation.Int.J.Curr.Microbiol.App.Sci. 6(12): 3808-3816. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.