Phoma cajani was isolated from stem canker infected stems and branches of pigeon pea. In this study the effects of culture media, temperature and pH on mycelial growth of Phoma cajani was evaluated. The six isolates of Phoma cajani were grown on nine different natural, semi- synthetic and synthetic media viz. potato dextrose agar, chick pea seed agar, maize meal agar, nutrient agar, honey peptone agar, malt extract-peptone-dextrose agar, yeast extract-dextrose-agar, czapek-dox agar and martin’s agar to study the radial growth. In the present study based on their growth rate, among six isolates, P1 showed better growth on natural media than semi synthetic media followed by synthetic media as compared to remaining five isolates. The mycelial growth was highly affected with temperature. The fungus grew from 15 - 30oC, radial growth of the isolate P1 was found to be maximum at 25-30ºC and slow growth at 15oC and 18oC. The fungus grew at pH 4 – 8. The pH 6, most favorable pH followed by pH 7 and pH 5 was demonstrated in the present study.

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