In present study efforts were made to improve the regeneration protocol in the oat cultivars JO-1 and OS-6. In mature embryo and immature embryo, callus induction was observed maximum on MS medium supplemented with 2,4-D (4.0mg/L and 6mg/L) respectively whereas, in case of embryogenic calli MS medium supplemented with 2,4-D (6.0 mg/L) was found to be best and in organogenesis MS medium supplemented with BAP (2-4mg/L) and NAA (0.1mg/L) was found to be better. In case of leaf base, callus induction was observed maximum on MS medium supplemented with 2,4-D (2.0 mg/L) whereas, in case of embryogenic calli MS medium supplemented with 2,4-D (2.0 mg/L) was found to be better and in organogenesis MS medium supplemented with BAP (2-4 mg/L) and NAA (0.1 mg/L) was found to be better. Among different genotypes JO-1 produced maximum number of induced calli, embryogenic calli as well as organogenic calli. While, genotype OS-6 performed moderately for initiation of calli.

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