International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:11, November, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 4291-4296

Effects of Antioxidants in Controlling Phenolic Exudates in in vitro Culture of Gliricidia [Gliricidia sepium (Jacq.) Steud.]
Aparna1*, M.L. Jakhar1, Rahul Kumar Tiwari3 and Leela Bhatt2
1Department of Plant Breeding and Genetics
2Department of Horticulture, SKNAU Jobner, India
3Division of Plant Pathology, IARI, New Delhi, India
*Corresponding author

The present investigation was carried out to study the influence of antioxidants on in vitro culture of Gliricidia sepium. Soft nodal stem segments were inoculated on MS medium supplemented with desired concentrations plant growth regulator such as cytokinins (BAP/Kinetin) and different antioxidants. Antioxidants viz., activated charcoal, ascorbic acid, citric acid and polyvinylpyrollidone were used to control the accumulation of phenolic compounds in the culture medium and enhance the rate of micropropagation. All cultures were incubated at 25+20C under fluorescent light in a 14: 10 hour’s photoperiod. Maximum number of shoot bud induction was obtained when MS medium is supplemented with 0.5 mg/l and 250 mg/l ascorbic acid.

Keywords: Cytokinins, Antioxidant, Micro propagation and Gliricidia.

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How to cite this article:

Aparna, M.L. Jakhar, Rahul Kumar Tiwari and Leela Bhatt. 2017. Effects of Antioxidants in Controlling Phenolic Exudates in in vitro Culture of Gliricidia [Gliricidia sepium (Jacq.) Steud.].Int.J.Curr.Microbiol.App.Sci. 6(11): 4291-4296. doi:
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