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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:11, November, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 4140-4144
DOI: https://doi.org/10.20546/ijcmas.2017.611.485


Evaluation of Histopathological Changes and Enzyme Bioassay during Interaction between Verticillium fungicola and Agaricus bisporus
Jagdeep Singh*, Surjeet Singh, Manoj Kumar, Anil Kumar and Rakesh Kumar
Department of Plant Pathology, College of Agriculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar-125004, Haryana, India
*Corresponding author
Abstract:

Verticillium fungicola var. fungicola (Preuss) is the most important dry bubble disease causing pathogen of the Agaricus bisporus (Lange) Imbach. Therefore, present investigation carried out on A. bisporus host and V. fungicola pathogen; by covering an aspect of interaction, variability and management of Verticillium dry bubble disease in India. Host and pathogen easily distinguishable at interaction regions on the basis of hyphal width i.e. ranged from 4.0 to 6.5 µm and 1.5 to 4.5 µm respectively. Pathogen grow inter and interacellularly on host hyphae and resulting in damaged, coiling and lysis of host mycelia. During enzymatic bioassay in dual culture on inducing substrate and mushroom extracts, mycopathogen showed the production of different hydrolytic enzymes i.e. amylase, cellulose and chitinase but not lipase and pectinase by formation of clear zonation on substrate.


Keywords: Verticillium fungicola, Agaricus bisporus, Enzyme, Interaction, Histopathology.

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How to cite this article:

Jagdeep Singh, Surjeet Singh, Manoj Kumar, Anil Kumar and Rakesh Kumar. 2017. Evaluation of Histopathological Changes and Enzyme Bioassay during Interaction between Verticillium fungicola and Agaricus bisporus.Int.J.Curr.Microbiol.App.Sci. 6(11): 4140-4144. doi: https://doi.org/10.20546/ijcmas.2017.611.485
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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