International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:11, November, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 3457-3469

Cultural and Morphological Characterization of Rhizoctonia solani f. sp. sasakii Isolates Collected from Different Districts of Andhra Pradesh
Bindu Madhavi Gopireddy1*, G. Uma Devi2, K.Vijayakrishna Kumar1, T. Ramesh Babu1 and T.C.M.Naidu1
1Acharya N G Ranga Agricultural University, Guntur, Andhra Pradesh, India
2Professor Jayasankar Telangana State Agricultural University, Hyderabad, Telangana, India
*Corresponding author

Sixty BLSB affected maize samples were collected from three districts and pathogen was isolated and identified based on morphological, cultural and sclerotial characters using standard descriptions of IMI. Light microscopic studies revealed that all the isolates of R. solani f. sp. sasakiiare characteristically branched out at right angle in the distal end of the cell and showed a characteristic constriction at the point of branching. Formation of septum near the point of origin of the branch / adjacent to branch was present in most of the isolates except for isolates RS 44, RS 48, RS 58 and RS 59, where in the septum was slightly away from the origin of branching. The hyphal width of all the 60 isolates varied from 5.05 μm (RS 52) to 7.98 μm (RS 15). Out of 60 maize isolates, eight isolates i.e., RS 7, RS 8, RS 9, RS 10, RS 11, RS 12 RS 16 and RS 26 produced barrel shaped monilliod cells and the remaining isolates produced irregular shaped monilliod cells. Clamp connections were absent in all the isolates, multinucleate and the number of nuclei per cell varied from five to seven in the isolates.

Keywords: Maize, BLSB, Rhizoctonia solani f. sp. sasakii, Morphological, Cultural characterization.

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How to cite this article:

Bindu Madhavi Gopireddy, G. Uma Devi, K.Vijayakrishna Kumar, T. Ramesh Babu and Naidu, T. C. M. 2017. Cultural and Morphological Characterization of Rhizoctonia solani f. sp. sasakii Isolates Collected from Different Districts of Andhra Pradesh.Int.J.Curr.Microbiol.App.Sci. 6(11): 3457-3469. doi:
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