International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:11, November, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 3405-3411

Identification of Rice Hybrids and Restorer Line Using Microsatellite Markers
Alice Xalxo, Prabha R. Chaudhari*, Deepak Sharma, Ritu R. Saxena, Satyapal Singh and AashishTiwari
Department of Genetics and Plant breeding, College of Agriculture Raipur, IGKV, (C.G.), India
*Corresponding author

Twenty four hybrids developed from three CMS lines viz. IR79156A, CRMS 31A and CRMS 32A and eight diverse rice genotypes were included in the present study. Molecular marker assisted identification with high power of genetic resolutions has emerged as a robust technique for cultivar fingerprinting, identity profiling, estimating and comparing genetic similarity, and variety protection. Out of sixteen, ten SSR markers produced polymorphic bands in the rice genotypes.RM171 and RM 216 were successfully recognized as hybrid and was discriminated from its parental lines (with same alleles).PCR based markers RM171 linked to Rf4 respectively, found to be more accurate compared to other linked markers in identifying restorers and differentiating maintainers from others. Molecular screening with RM171 for fertility restoration can be a useful tool for identifying restorers from breeding lines of unknown restoration status with 100% efficiency without making and evaluating large number of test crosses. These markers are useful in marker assisted identification of Rf genes in back cross breeding program to develop near isogenic lines with multiple Rf genes towards the development of superior restorer lines.

Keywords: Rice, Identification Restorers, Molecular Marker, Rf4.

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How to cite this article:

Alice Xalxo, Prabha R. Chaudhari, Deepak Sharma, Ritu R. Saxena, Satyapal Singh and Aashish Tiwari. 2017. Identification of Rice Hybrids and Restorer Line Using Microsatellite Markers.Int.J.Curr.Microbiol.App.Sci. 6(11): 3405-3411. doi:
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