National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Twenty four hybrids developed from three CMS lines viz. IR79156A, CRMS 31A and CRMS 32A and eight diverse rice genotypes were included in the present study. Molecular marker assisted identification with high power of genetic resolutions has emerged as a robust technique for cultivar fingerprinting, identity profiling, estimating and comparing genetic similarity, and variety protection. Out of sixteen, ten SSR markers produced polymorphic bands in the rice genotypes.RM171 and RM 216 were successfully recognized as hybrid and was discriminated from its parental lines (with same alleles).PCR based markers RM171 linked to Rf4 respectively, found to be more accurate compared to other linked markers in identifying restorers and differentiating maintainers from others. Molecular screening with RM171 for fertility restoration can be a useful tool for identifying restorers from breeding lines of unknown restoration status with 100% efficiency without making and evaluating large number of test crosses. These markers are useful in marker assisted identification of Rf genes in back cross breeding program to develop near isogenic lines with multiple Rf genes towards the development of superior restorer lines.
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