National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study aimed at characterizing the quorum sensing loci of potential Bacillus strain from pigeonpea rhizosphere for combating the root rot and wilt diseases of pigeonpea. Screening of 139 Bacillus isolates from pigeonpea rhizosphere under in vitro conditions revealed that 18 were potential inhibitors of root rot and wilt pathogens. Formation of biofilm by these isolates was observed in solid and liquid media which was quantified by microtitre plate assay. The presence of quorum sensing genes (comQ, comX, comP and comA) in Bacillus strains were detected by PCR following primer designing using the Primer3 software. The ComQXP quorum sensing loci was amplified at approximately 3 kb using forward primer UniComQ and the reverse primer P1. The protein translates of the comX and comP regions of the sequenced nucleotides revealed identity with comX pheromone precursor and histidine kinase sensor protein respectively. The ComA gene of CcB7 was sequenced and the protein translates of which showed identity to response regulation transcription factor. All the sequences are deposited at the GenBank Database.
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