National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Survey was conducted over six major potato growing districts of Punjab during November to December, 2015 to investigate the occurrence of Cucumber mosaic virus on potato. Leaf samples of total 95 potato plants showing various viral diseases like symptoms were collected and exposed against antisera of 10 viruses in DAS/TAS-Enzyme Linked Immunosorbent Assay (ELISA).Ten potato samples (10.53 %) showed positive reactions with antisera of CMV in ELISA. The prevalence of CMV sub group I was more (8.42 %) as compared to CMV sub group II (6.32 %) over potato crop in Punjab. Furthermore, successful amplification of approximately 540 bp region of CMV coat protein by RT-PCR confirmed the presence of CMV in the potato samples collected. Variable symptoms including mosaic, yellowing, mild blistering, wavy leaf margin, malformation of leaves, curling, stunting and reduced leaf size were induced by CMV infection on potato. CMV was mechanically transmitted (60%) from infected potato to muskmelon. The aphids viz. Myzus persicae and Aphis gossypii transmitted CMV from infected potato to muskmelon with an efficiency of 40 per cent under controlled conditions. The aphid population recorded highest in potato (245 aphids/ 100 compound leaves) and in muskmelon (75 aphids/10 vines) during the second fortnight of February. In Punjab, the commonly followed cropping pattern is cultivation of potato from October to February followed by the cultivation of cucurbits from February to May. Hence, the aphids are acquiring Cucumber mosaic virus from infected potato plants and transmitting the virus into healthy muskmelon plants mainly during the months of February and March.
Citations |
 |
 |
 |
 |
