National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Chilli (Capsicum annuum L.) is an important crop grown worldwide for its use as spice and vegetable. Murda complex is the most destructive syndrome causing substantial economic losses in chilli production. Severe leaf curling, leaf distortion, vein banding, reduced leaf size and stunted growth are typical symptoms of the viral infection. Chilli is known to be infected by many viruses and association of Geminivirus with murda complex is under question as the complex disease doesn’t associate with whiteflies. For a reliable identification of virus associated with murda complex disease in chilli (Cv. Byadgi Dabbi), gene specific primer pairs were designed for Potyvirus (CVMV), Geminivirus (ToLCV), Tospovirus (GBNV), TMV and CMV. Upon amplification by PCR using CVMV gene specific primers, ~531 bp size amplicon was amplified in all diseased samples of chilli and no amplification was obtained in healthy samples of chilli. However, no amplification was found with ToLCV, GBNV, TMV and CMV gene specific primer pairs. Thus, the investigation focused on molecular identification, convincingly revealed Chilli veinal mottle virus (Potyvirus) association with the chilli murda complex.
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