International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:11, November, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 2780-2796

Recent Studies on N2 Fixing Burkholderia Isolates as a Biofertilizer for the Sustainable Agriculture
A. Sandanakirouchenane1*, Ekramul Haque1 and T. Geetha2
1Department of Microbiology, School of Life Sciences, Pondicherry University,Pondicherry-605014, India
2Department of Agricultural Microbiology, Adhiparasakthi agricultural college,
Kalavai-632506, India
*Corresponding author

In this study thirteen isolates of Burkholderia sp. were isolated from the root, stem and leaf samples of four different crops viz., rice, maize, sugarcane and blackgram, using N-free Burkholderia Azelaic acid (BAz) semi solid medium. Based on growth performance on different media the following isolates viz., RB1 (Rice Burkholderia 1), MB2 (Maize Burkholderia 2), SB3 (Sugarcane Burkholderia 3) and BB4 (Black gram Burkholderia 4) were selected for further studies. Among the four isolates, BB4 isolate showed the maximum nitrogenase activity (81.74 n moles ethylene produced h-1 /mg of cell protein) followed by SB3, RB1 and MB2. Interestingly the cell protein content (1.54 mg g-1), ammonia excretion (10.04 g ml-1), polysaccharide production (28.82 mg g-1) and hydrogen cyanide production (44.42 g ml-1) were also observed more in BB4 than others. Further the Pot culture experiment was carried out to evaluate the effect of best N2 fixing selected isolate (BB4) on the growth of maize. The maximum germination percentage (83.3 per cent) was observed in the maize when it was treated with Burkholderia + 100% N + P fertilizer. At the same time after 60th day of sowing higher shoot (59.7cm) and root (32 cm) growth of maize was observed when it was treated with Burkholderia + 100 % N + P, which was on par with the treatment with Azospirillum + Phosphobacteria +100 % N and P. Therefore it is clear from the above observation that Burkholderia sp (BB4) may be useful as a cost effective bio fertilizer to promote sustainable agriculture.

Keywords: Burkholderia sp, Physiology, Nitogenase activity, Biofertilizer, Pot culture.

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Sandanakirouchenane, A., Ekramul Haque and Geetha T. 2017. Recent Studies on N2 Fixing Burkholderia Isolates as a Biofertilizer for the Sustainable Agriculture.Int.J.Curr.Microbiol.App.Sci. 6(11): 2780-2796. doi:
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