National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study presents a rapid efficient DNA extraction protocol for rice and tomato crops. Five published protocols (Doyle & Doyle CTAB Procedure, Urea Method, Salt extraction method, IRRI Method, Dellaporta DNA Extraction method) were comparatively evaluated in rice and tomato crops for DNA purity and yield. The analysis of variance revealed that the yield was significantly different (P < 0.01) for all protocols in both crops, whereas there was no significant observation was observed for purity. The multiple Duncan test revealed that, Doyle & Doyle CTAB Procedure was best for rice crop (2481 ng/µl), whereas urea method was best for tomato crop (2685 ng/µl). Here Proposed that urea method, Doyle & Doyle CTAB Procedure is best method for good quality DNA and higher yield in tomato and rice crop respectively. Hence this method of extraction would be ideal for genotyping of large populations of rice and tomato genotypes.
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