National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of the present investigation was to study the effect of soybean leaf protein and protease inhibitor content on the mean leaf area consumed by Spodoptera litura and Spilosoma obliqua larva. The activity of trypsin inhibitor was assayed by determining the residual trypsin activity by using BapNA as the substrate and bovine trypsin as the standard enzyme. The highest and lowest TIA activity exhibited by genotypes SL 979 and SL 688 (2.25 TUI.mg-1protein) and CSB 904 (0.35 TUI.mg-1protein), respectively. The highest gut trypsin inhibition activity ascertained in larvae fed on SL 688 (14.40 %) While the lowest intensity of gut trypsin inhibition activity was observed in genotypes NRC 94 (0.12%). A highly significant and negative correlation was observed between MLAC (cm2) by S. litura and S. obliqua and protein content in 33, leaves (r= -0.728**) and (-0.674**) respectively and trypsin inhibitor in leaves (r= -0.909**) and (r= -0.913**). Thus it can be concluded that the genotypes which were having higher protein and trypsin inhibitor in their leaves offered resistance against S. litura and S. boliqua in soybean.
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