National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Lipolytic enzymes are currently attracting significant attention because of their biotechnological potential. Most of the lipases used in industry are microbial enzymes, of both fungal and bacterial origin. Windrow composting is a controlled, self-heating, aerobic degradation of organic materials by mesophilic and thermophilic microorganisms. The present study deals with isolation of lipase producing bacteria from windrow compost. The samples were collected from compost heap at an interval of 10 days and screened for lipase producing bacterial fauna in Tributyrin agar medium after serial dilution. A clear zone of hydrolysis indicated enzyme production. Out of 73 bacterial colonies screened, 24 lipase producing bacterial strains were isolated by checking the production of zone clearance in the tributyrin agar medium. Based on the morphological and biochemical characteristics 24 bacterial strains were identified up to species levels with the help of ABIS online analysis tool. The best lipase producers Staphylococcus (WCS1C2) and Pseudomonas (WCS3C2) genus were further characterized by 16s rDNA sequencing and identified as Staphylococcus saprophytics and Pseudomonas otidis. These bacterial strains can be used for the production of lipase enzyme for industrial applications.
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