National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The use of plant tissue culture as a routine method of potato seed production would be costly but these techniques can be used to eliminate the pathogens, produce required initial material and then, use another efficient and cheaper system to rapidly produce high quality seed tubers for commercial production. Considering this, the liquid formulation of antimycotic 100x, and amphotericin-B 250 µg/ml of PAA make was used. These test antibiotics were added aseptically at various concentrations (0.5%, 1%, 1.5%, and 2%) in the sterilized MS medium culture tubes to find out contamination free, vigorous in-vitro growth of the microplants. It was observed that amphotericin-b at lower conc. i.e., 0.5% and 1% could be incorporated in the micro propagation medium of potato to minimize fungal contaminations as well as for improving the microplants vigour. However, all the tested antimycotic concentrations had negative effects on almost all the morphological characters studied, which need further investigation with lower concentrations.
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