National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The consumption of fresh and minimally processed vegetables is considered healthy, outbreaks related to the contamination of these products are frequently reported. Present study aimed to evaluate the microbiological quality and the occurrence of A. hydrophila in external, internal and macerated part of salad vegetables (cucumber, radish, carrot, tomato, cabbage, long melon and spinach) from the fields of Punjab Agricultural University and local markets of Ludhiana. Epidemiological surveillance conducted showed the occurrence of A. hydrophila in 82.5% of total tested samples, cucumber (80%), radish (83%), cabbage (100%), carrot (74%), long melon (85%), spinach (100%) and tomato (68.5%). Total plate count ranged from 4.82 to 6.25 log cfu/g. Aerobic plate count of A. hydrophila procured from field and local market ranged between 2.79-3.93 log cfu/g, A. hydrophila count from internal, external and macerated part was 2.54, 3.06 and 3.73 log cfu/g. Isolate were molecularly confirmed as A. hydrophila by 16s -rDNA specific primer. Virulence was confirmed by gene specific primers, act (232 bp) and ahh (130 bp). Results of the study showed that salad vegetables possess a potential risk for the consumers.
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