International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:5, May, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 864-869

Detection of Carbapenem Resistant Enterobacteriacae from Clinical Isolates
Akshaya Rao* and V.A. Indumathi
Department of Microbiology M.S. Ramaiah Medical College Bangalore, India
*Corresponding author

Carbapenemases are β-lactamases that confer resistance to almost all β-Lactam antibiotics. There is an urgent need for identification of these enzymes. The main aim of this study includes to detect different Carbapenemase enzymes produced by Enterobacteriacae among clinical isolates using phenotypic tests. Isolates from various clinical samples that showed resistance to Imipenem and/or Meropenem by Kirby Bauer disc diffusion method were analysed over a period of 6 months. These isolates were screened using Modified Hodge test (MHT), KPC CHROM agar, and supplemental tests such as Blue Carba Test (BCT) and Inhibitor Based Phenotypic Test (IBPT) were done to detect different Carbapenemase enzymes.  60 out of 430 (13.95%) strains were Carbapenem resistant Enterobacteriacae. Among the 4 tests performed MHT detected Carbapenemases in 36.6% and KPC CHROM agar in 96.6% isolates. Blue Carba Test showed 13.3% isolates as KPC/MBL phenotype and 3.3% as OXA phenotype. Inhibitor Based Phenotypic Test detected 8.3% isolates as KPC producers and 5% as MBL producers. Among these four phenotypic tests KPC CHROM agar could be useful as a screening test for Carbapenemase detection, BCT and IBPT would be useful to differentiate these Carbapenemases.

Keywords: Carbapenemases, KPC Chrom Agar, Modified Hodge Test,Blue Carba Test,Inhibitor Based Phenotypic Test.

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Carbapenemases, KPC Chrom Agar, Modified Hodge Test,Blue Carba Test,Inhibitor Based Phenotypic Test.Int.J.Curr.Microbiol.App.Sci. 5(5): 864-869. doi:
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