International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:5, May, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 848-854

Isolation of Group B Streptococci from Antenatal Women
Tupili Ramya, Usham Gangaram* and K.H. Vasudeva Naidu 
Department of Microbiology, Sri Vekateshwara Medical College, Tirupathi, India
*Corresponding author

Prevalence of Group-B streptococcal (GBS) infection varies from place to place and this organism is responsible for serious infections in newborns such as septicemia and meningitis. The present study was carried out to find out the prevalence & antibiotic sensitivity of GBS in primigravida & to identify the risk factors. 300 pregnant women were studied attending the antenatal clinics in Government Maternity Hospital, Tirupati. Two vaginal swabs were taken from each pregnant women. The two swabs were immediately transported to the laboratory for processing. Direct Gram stain was done from one swab and the other swab was inoculated onto sheep blood agar plate and incubated at 37ºC for 24-48 hours. Identification was done based on Gram staining, colony morphology, catalase reaction, CAMP test and Hippurate hydrolysis test & Bacitracin resistance. Of the 300 pregnant women screened, 7% were colonized by GBS. GBS colonization rate was higher among pregnant women in third trimester who were < 25 years of age. All the isolates were sensitive to Penicillin, Ampicillin, ceftriaxone, levofloxacin and vancomycin. GBS colonization rate among pregnant women in third trimester of pregnancy is low in this area. Revised guidelines from the Centers of Disease Control and Prevention (CDC), 2010 for the prevention of perinatal GBS disease recommends that all pregnant women be screened for GBS carriage between 35 and 37 weeks of gestation and intrapartum antibiotic prophylaxis be given to colonized women at the time of labour onset or rupture of membranes.

Keywords: Group B streptococcus, Colonization,Drug resistance, Drug sensitivity.

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How to cite this article:

Tupili Ramya, Usham Gangaram and Vasudeva Naidu, K.H. 2016. Isolation of Group B Streptococci from Antenatal Women.Int.J.Curr.Microbiol.App.Sci. 5(5): 848-854. doi:
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