Mathura oil refinery is one of the major oil refineries in India, situated near the Mathura, Uttarpradesh. Hence nearby area of this region was chosen for investigation of soil pollution caused by Crude oil and its counterparts. Soil samples were characterized for various physico-chemical parameters via standard analytical methods. In present investigation augmented values of  available Chloride, Bicarbonate, Organic Carbon and Organic Matter, Electric Conductivity,  C/N ratio were observed in contaminated samples as compared to control samples. While declined values of pH and WHC were recorded in contaminated samples. Higher content of micronutrients was also observed in polluted samples than uncontaminated one. These accumulated heavy metals adversely distress the ecosystem, both soil and water quality and ultimately harm to health of both flora and fauna by food chain. As hydrocarbon contamination has altered the physical parameters of soil, consequently microbial status of the site is also affected. The indigenous microbial colony was decreased in contaminated samples while petroleum degrading population was found in increased manner as compared to control soil sample.

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