A retrospective study was done and culture and sensitivity reports were collected from RIMS, Teaching Hospital, Central Laboratory Microbiology Department, Raichur for a period of Sep 2014 to April 2016 were analysed. A total of 351 urine samples collected during a time period of 20 months from September 2014 to April 2016 and fulfilling the inclusion criteria were included in the study. The objective was to assess the distribution of urinary tract bacterial pathogens and to determine the antibiotic susceptibility profile of the isolates in our setting. Single positive cultures with a colony count of >105 CFU on CLED medium were identified and their antibiotic sensitivity and resistance profile was depicted in the form of an antibiogram. Out of 351 urine samples 217(61.8%) urine samples were culture positive with significance. 57.1% of the total isolates were identified as E. coli, followed by Pseudomonas spp. 16.1%, Klebsiella pneumonia 9.6%, Proteus spp 4.6%, Citrobacter spp 4.1%, Staphylococcus aureus 4.6%, Coagulase negative Staphylococcus 2.3% and Candida spp 1.4%. Among 199 gram negative organism isolated it was observed that 198/199(99.4%) of total tested isolates were sensitive to Imipenem followed by Piperacillin/Tazobactam 170/199(85.4%), Cefoperazone/sulbactam 128/199(64.3%), Ceftazidime 112/199(56.2%), Augmentin 107/199(53.7%), Amikacin 105/199(52.7%), Nitrofuration 101/199(50.7%), Cefipime 100/199(50.2%), Ceftriaxone 95/199(47.7%), Gentamicin 90/199(45.2%), Nalidixic acid 75/199(37.6%), Levofloxacin 70/199(35.1%), Ciprofloxacin 48/199(24.1%), Cotrimoxazole 28/199(14.1%). Also out of 18 gram positive organisms isolated 3 were Candida species. Sensitivity profile of gram positive organisms showed Vancomycin was most susceptible 18/18(100%) followed by Augmentin 14/18(77.7%), while Penicillin was least susceptible 4/18(22.2%). This is an alarmingly low sensitivity to Ciprofloxacin, Cotrimoxazole and Penicillin which is routinely used as empirical therapy for uncomplicated community acquired urinary tract infections and other infections. There is need for proper sensitivity testing to guide the management of UTIs with hospital antibiotic policies.

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